Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. The book comprises of 19 chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include Introduction, Basic Equipment and Facilities for Plant Tissue Culture, Tissue Culture Techniques, Plant Tissue Culture Media, Protoplast Isolation and Culture, Somatic Hybridization and Cybridization, Somatic Embryogenesis, Synthetic Seed, Micropropagation and Organogenesis, Haploid Production, Triploid Production, Embryo Culture, Germplasm Conservation, Genetic Transformation, Genetically Modified (GM) Plants, Metabolic Engineering and Plant Cell Cultures, Plant Genetic Engineering and Speciation Genes in Plants, Molecular Markers and their Applications in Plant Species, Glossary, Bibliolgraphy. The present book is an endeavour to make comprehensive study material related to Plant Tissue Culture, accessible to the readers. This book has been compiled to accomplish the needs of undergraduate and post-graduate students of Plant Tissue Culture. Faculty, graduate students, and undergraduates interested in plant cell and tissue culture find this book is indispensable. Aspirants for the Civil Services and other competitive examinations too will surely gain advantage by this book.

Dr. B.S. Jamwal, Chief Scientist-cum-Professor (Plant Breeding & Genetics) & In-charge, Pulses Research Sub-Station, Samba, SKUAST-Jammu. He did his M.Sc (Agriculture) from SKUAST, Srinagar & Ph.D. in Plant Breeding & Genetics from SKUAST-Jammu. He is working as P.I., AICRP on Chickpea, Sub-Centre, Samba. He has vast research experience of past four decades in different pulse crops, maize and wheat crops and was associated with seed production activities and is presently working as Nodal Officer, Pulses Seed Hub Project of SKUAST-Jammu and actively engaged in research of Chickpea and other MULLaRP crops. He has also more than one decade of teaching experience and has guided many post graduate students of Plant Breeding. He is founder member of Indian Society of Pulses Research and Development and Fellow of ISPRD, ISG & PB, ISST, SAWR and Life member of many more Scientific Societies and Associations. He has been Councilor from North Zone for Indian Science Congress Association, Kolkota & ISPRD, I.I.P.R, Kanpur. He has many research publications in his credit.